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human il 6 elisa kit (R&D Systems)

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R&D Systems human il 6 elisa kit

Unadjusted exercise‐induced changes <t>in</t> <t>IL‐6</t> in males and females (Model 1). Estimated marginal means of IL‐6 derived from linear mixed‐effects Model 1 (without covariates) were back‐transformed from the log scale using the exponential function and are presented in the original units. Error bars represent 95% confidence intervals. These differences became nonsignificant in Model 2, in which mean power output during exercise was included as a covariate. Sample size: MP and LP, n = 12. LP, luteal phase; MP, menstrual phase.

Human Il 6 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantikine+hs+human+il+6+elisa+kit/Human+IL-6+Quantikine+HS+ELISA/pmc13051937-77-8-14
Average 96 stars, based on 808 article reviews

human il 6 elisa kit - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Sex differences in IL ‐6 responses to moderate‐intensity aerobic exercise are explained by cycling power output"

Article Title: Sex differences in IL ‐6 responses to moderate‐intensity aerobic exercise are explained by cycling power output

Journal: Physiological Reports

doi: 10.14814/phy2.70850

Unadjusted exercise‐induced changes in IL‐6 in males and females (Model 1). Estimated marginal means of IL‐6 derived from linear mixed‐effects Model 1 (without covariates) were back‐transformed from the log scale using the exponential function and are presented in the original units. Error bars represent 95% confidence intervals. These differences became nonsignificant in Model 2, in which mean power output during exercise was included as a covariate. Sample size: MP and LP, n = 12. LP, luteal phase; MP, menstrual phase.

Figure Legend Snippet: Unadjusted exercise‐induced changes in IL‐6 in males and females (Model 1). Estimated marginal means of IL‐6 derived from linear mixed‐effects Model 1 (without covariates) were back‐transformed from the log scale using the exponential function and are presented in the original units. Error bars represent 95% confidence intervals. These differences became nonsignificant in Model 2, in which mean power output during exercise was included as a covariate. Sample size: MP and LP, n = 12. LP, luteal phase; MP, menstrual phase.

Techniques Used: Derivative Assay, Transformation Assay

Clinical Proteomics:

Article Title: Inflammation and oxidative stress markers and esophageal adenocarcinoma incidence in a Barrett’s esophagus cohort
Article Snippet: .. IL-6 was assayed in never-thawed plasma samples using the Quantikine HS human IL-6 Elisa kit (R&D Systems Inc, Minneapolis, MN; HS600B) [Inter-batch CV 4.4%, intra-batch CV 4.1%, ICC(95% CI) 56.4% (49.4,63.4)]. .. Samples were run in duplicate with a median duplicate CV of 2.7%; samples with CVs greater than 12% were re-run and the repeat measurements used for analysis. sTNFR-I and sTNFR-II were measured on previously thawed and re-frozen plasma samples using the MILLIPLEX MAP Human Soluble Cytokine Receptor Panel (Millipore, Billerica, MA; HSCR-32K) [sTNFR-I: inter-batch CV 8.9%, intra-batch CV 5.9%, ICC(95% CI) 89.2% (87.2,91.3); sTNFR-II: inter-batch CV 6.1%, intra-batch CV 2.4%, ICC(95% CI) 84.9% (82.1,87.8)].

Article Title: A Combination of Low-Dose Fluvastatin and Valsartan Decreases Inflammation and Oxidative Stress in Apparently Healthy Middle-Aged Males
Article Snippet: ■ METHODS: The study was performed supplementary to 3 previous studies, overall 65 treated participants (25 received fluvastatin 10 mg, 20 valsartan 20 mg, 20 their combination) and 65 participants placebo.. The stored blood samples (collected at inclusion and after 30 days of treatment) were used to measure high-sensitivity CRP, interleukin-6, vascular cell adhesion molecule-1, total antioxidant status, glutathione peroxidase, and selenium concentration.

Article Title: Intraindividual variability over time in plasma biomarkers of inflammation and effects of long-term storage
Article Snippet: .. Plasma levels of IL-6 were assayed using the Quantikine HS human IL-6 Elisa kit (R&D Systems Inc, HS600B) according to the manufacturer’s instructions. ..

Article Title: Proinflammatory Features of Stem Cell-like Memory T Cells from Human Patients with Rheumatoid Arthritis.
Article Snippet: .. Plasma was collected from RA patients and HCs and stored at 80 C. Plasma IL-6 levels were measured with the Quantikine HS Human IL-6 ELISA kit (R&D Systems, Minneapolis, MN). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Interferon autoantibodies associated with AIRE deficiency decrease the expression of IFN-stimulated genes
Article Snippet: Primers for all target genes and housekeeping β-actin (ACTb) gene were designed to span exon boundaries in all multiexon genes with Primer Express (Applied Biosystems) and are listed in Table S1 (available on the Blood website; see the Supplemental Materials link at the top of the online article). .. IP-10 (CXCL10) Duo Elisa kit and Quantikine HS human IL-6 ELISA kit (both from R&D Systems) were used to detect their concentrations in patient and control sera. .. Human Th1/Th2 Cytokine Kit (Cytometric Bead Array, BD Biosciences) was used according to the manufacturer's instructions to measure IFN-γ, tumor necrosis factor (TNF)-α, IL-10, IL-5, IL-4, and IL-2 concentrations in patient and control serum samples with the help of FACSCalibur.

Immunocytochemistry:

Control:

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Journal: Physiological Reports

Article Title: Sex differences in IL ‐6 responses to moderate‐intensity aerobic exercise are explained by cycling power output

doi: 10.14814/phy2.70850

Figure Lengend Snippet: Unadjusted exercise‐induced changes in IL‐6 in males and females (Model 1). Estimated marginal means of IL‐6 derived from linear mixed‐effects Model 1 (without covariates) were back‐transformed from the log scale using the exponential function and are presented in the original units. Error bars represent 95% confidence intervals. These differences became nonsignificant in Model 2, in which mean power output during exercise was included as a covariate. Sample size: MP and LP, n = 12. LP, luteal phase; MP, menstrual phase.

Article Snippet: IL‐6 concentrations were measured in serum using a human IL‐6 ELISA kit (Quantikine HS600C, R&D Systems, Minneapolis, MN, USA). d‐ROMs were measured in serum using a commercial kit (d‐ROMs Test Kit, DI‐003b, Wismerll Co., Ltd., Tokyo, Japan).

Techniques: Derivative Assay, Transformation Assay

a Scatterplot comparing effect sizes from the base model (unadjusted) versus a model adjusted for twelve predicted blood cell-type proportions. CpG sites with insufficient evidence for an independent IL-6 effect ( p fdr ≥ 0.05) after adjustment are highlighted in yellow ( n = 130). b Scatterplot comparing base model effect sizes versus a model adjusted for smoking status. CpGs previously removed due to cell-type confounding are shown in grey and one CpG removed due to smoking effects is highlighted in yellow. Data underlying Fig. 2 is provided in Supplementary Data 02 .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Scatterplot comparing effect sizes from the base model (unadjusted) versus a model adjusted for twelve predicted blood cell-type proportions. CpG sites with insufficient evidence for an independent IL-6 effect ( p fdr ≥ 0.05) after adjustment are highlighted in yellow ( n = 130). b Scatterplot comparing base model effect sizes versus a model adjusted for smoking status. CpGs previously removed due to cell-type confounding are shown in grey and one CpG removed due to smoking effects is highlighted in yellow. Data underlying Fig. 2 is provided in Supplementary Data 02 .

Article Snippet: Serum concentrations of IL-6 were measured with the Human IL-6 Quantikine HS ELISA kit (R&D Systems, Wiesbaden, Germany), and hsCRP was determined by a high-sensitivity latex-enhanced nephelometric assay on a BN II analyzer (Dade Behring) .

Techniques:

a Directional Manhattan plot showing the genomic position of all 412,226 tested CpGs ( x -axis) and their signed log 10 p -value ( y -axis). CpGs positively associated with IL-6 are displayed in the upper half, and inversely correlated CpGs in the lower half. The 401 CpGs associated with IL-6 after multiple testing correction and sensitivity analyses are highlighted in blue (odd chromosomes) and yellow (even chromosomes), while grey dots represent all other tested CpGs. b Effect sizes and 95% confidence intervals (CIs) for the 401 IL-6-associated CpGs, with points coloured by mean DNAm level. c Volcano plot showing the effect size ( x -axis) against −log 10 p -value ( y -axis) for all tested CpGs. Significant CpGs are coloured by mean DNAm level and the top hits are labelled, while all other CpGs are shown in grey. Data underlying Fig. 3 is provided in Supplementary Data 01 .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Directional Manhattan plot showing the genomic position of all 412,226 tested CpGs ( x -axis) and their signed log 10 p -value ( y -axis). CpGs positively associated with IL-6 are displayed in the upper half, and inversely correlated CpGs in the lower half. The 401 CpGs associated with IL-6 after multiple testing correction and sensitivity analyses are highlighted in blue (odd chromosomes) and yellow (even chromosomes), while grey dots represent all other tested CpGs. b Effect sizes and 95% confidence intervals (CIs) for the 401 IL-6-associated CpGs, with points coloured by mean DNAm level. c Volcano plot showing the effect size ( x -axis) against −log 10 p -value ( y -axis) for all tested CpGs. Significant CpGs are coloured by mean DNAm level and the top hits are labelled, while all other CpGs are shown in grey. Data underlying Fig. 3 is provided in Supplementary Data 01 .

Techniques:

a Scatterplot comparing IL-6 associated DNAm effect sizes before and after adjustment for hsCRP levels. b Scatterplot comparing hsCRP-associated DNAm effect sizes before and after adjustment for IL-6 levels. The Pearson correlation coefficient (R) and associated p -value are shown in blue for both plots. Data underlying Fig. 4 is provided in Supplementary Data 01, 03, and 04 .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Scatterplot comparing IL-6 associated DNAm effect sizes before and after adjustment for hsCRP levels. b Scatterplot comparing hsCRP-associated DNAm effect sizes before and after adjustment for IL-6 levels. The Pearson correlation coefficient (R) and associated p -value are shown in blue for both plots. Data underlying Fig. 4 is provided in Supplementary Data 01, 03, and 04 .

Techniques:

a Forest plot of ORs and 95% CIs with enrichment across 14 chromatin states, calculated using the Roadmap PBMC reference epigenome (E062) with all tested CpGs as the background set. States are sorted by OR, with blue denoting enrichment, yellow indicating depletion, and grey for non-significant results. One non-significant term (ZNF/Rpts) not shown due to an extremely wide 95% CI. b Bar plot of −log 10 p -values from TFBS enrichment analysis, performed using HOMER on sequences within 50 bp of IL-6 associated CpGs, tested against a GC-matched random genomic background. Data underlying Fig. 6 is provided in Supplementary Data 06 and 08 .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Forest plot of ORs and 95% CIs with enrichment across 14 chromatin states, calculated using the Roadmap PBMC reference epigenome (E062) with all tested CpGs as the background set. States are sorted by OR, with blue denoting enrichment, yellow indicating depletion, and grey for non-significant results. One non-significant term (ZNF/Rpts) not shown due to an extremely wide 95% CI. b Bar plot of −log 10 p -values from TFBS enrichment analysis, performed using HOMER on sequences within 50 bp of IL-6 associated CpGs, tested against a GC-matched random genomic background. Data underlying Fig. 6 is provided in Supplementary Data 06 and 08 .

Techniques:

a Top five enriched gene sets, plus eleven enriched terms with specific relevance to Th17 activation and IL-6 signalling. Bars are colored by the percentage of overlapping genes; genes mapped onto the aetiological cascade from T-cell activation to glycolysis-fuelled Th17 proliferation are indicated. b Top ten transcription factors (TFs) whose regulons are most enriched in the genes linked to IL-6-associated DNAm using DoRothEA TF target annotations. c Selected enriched terms (white boxes) and mapped genes (black boxes) arranged along the pathway to Th17 differentiation, including four activation signals: (i) TCR activation, (ii) co-stimulation, (iii) IL-1β stimulation, (iv) IL-6/JAK/STAT3 signalling, culminating in (v) mTORC1-driven glycolysis fuelling rapid proliferation and effector functions. Created in BioRender. Data underlying Fig. 7 is provided in Supplementary Data and .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Top five enriched gene sets, plus eleven enriched terms with specific relevance to Th17 activation and IL-6 signalling. Bars are colored by the percentage of overlapping genes; genes mapped onto the aetiological cascade from T-cell activation to glycolysis-fuelled Th17 proliferation are indicated. b Top ten transcription factors (TFs) whose regulons are most enriched in the genes linked to IL-6-associated DNAm using DoRothEA TF target annotations. c Selected enriched terms (white boxes) and mapped genes (black boxes) arranged along the pathway to Th17 differentiation, including four activation signals: (i) TCR activation, (ii) co-stimulation, (iii) IL-1β stimulation, (iv) IL-6/JAK/STAT3 signalling, culminating in (v) mTORC1-driven glycolysis fuelling rapid proliferation and effector functions. Created in BioRender. Data underlying Fig. 7 is provided in Supplementary Data and .

Techniques: Activation Assay

a Scatter plot comparing the observed effect (PGS-DNAm associations) with the predicted effect (combining PGS effect on IL-6 and IL-6 CpG associations). Correlation coefficients (R) and associated p -values are shown in blue. b Scatter plot comparing the observed (mQTL IL-6 associations) and predicted effects (combined effect of SNP on DNAm, and DNAm on IL-6). Each point represents an IL-6-associated CpG. Data underlying Fig. 8 is provided in Supplementary Data and .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Scatter plot comparing the observed effect (PGS-DNAm associations) with the predicted effect (combining PGS effect on IL-6 and IL-6 CpG associations). Correlation coefficients (R) and associated p -values are shown in blue. b Scatter plot comparing the observed (mQTL IL-6 associations) and predicted effects (combined effect of SNP on DNAm, and DNAm on IL-6). Each point represents an IL-6-associated CpG. Data underlying Fig. 8 is provided in Supplementary Data and .

Techniques:

a Forest plot showing the 52 genes linked to IL-6-associated DNAm that were responsive to IL-6 stimulation in isolated immune cells in vitro. b Forest plots of CpGs ranked in the top ten for at least one tested trait in the mediation analysis. Bars are coloured by the direction and significance of the mediated effect: orange for significant positive mediation, blue for significant negative mediation, and grey for non-significant findings after correcting for multiple testing. Data underlying Fig. is provided in Supplementary Data and .

Journal: Communications Biology

Article Title: Epigenome-wide association study of circulating interleukin-6 connects DNA methylation to immunometabolic and inflammatory health

doi: 10.1038/s42003-026-09520-2

Figure Lengend Snippet: a Forest plot showing the 52 genes linked to IL-6-associated DNAm that were responsive to IL-6 stimulation in isolated immune cells in vitro. b Forest plots of CpGs ranked in the top ten for at least one tested trait in the mediation analysis. Bars are coloured by the direction and significance of the mediated effect: orange for significant positive mediation, blue for significant negative mediation, and grey for non-significant findings after correcting for multiple testing. Data underlying Fig. is provided in Supplementary Data and .

Techniques: Isolation, In Vitro

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human il 6 elisa kit (R&D Systems)

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1) Product Images from "Sex differences in IL ‐6 responses to moderate‐intensity aerobic exercise are explained by cycling power output"

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